Coding

Part:BBa_K1021002:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-08-30)


hph (hygromycin phosphotransferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was extracted from its original vector and inserted into pSB1C3. Two illegal cutsites (EcoRI and PstI) were subsequently removed via site-directed mutagenesis.

Source

http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/

References